Calcium-induced peanut protein nanoparticles for resveratrol delivery
Currently, biodegradable nanoparticles derived from natural compounds are widely used in the biomedical field. In our previous work, the crosslinked-starch nanoparticles were developed. The release behavior of ciprofloxacin was confirmed to be a double decay exponential model (R2 N 0.97) and the drug release rate was dependent on the loading and drying methods.
Based on this thoughtfulness, calcium-induced peanut protein nanoparticles (PPN) were prepared with Z-average diameter of 94.66 ± 0.53 nm . The PPN exhibited uniform size distri-bution, spherical shape and excellent thermal, pH, dilution stability. The cytotoxicity assays indicated nontoxicity of PPN for human normal cells (LO2 liver cells and GES1 stomach cells). With res-veratrol as a model drug, the drug-loaded PPN has 82.7% of encapsulation efficiency and exhibited superior stability under extracellular conditions. Moreover, the photostability of resveratrol was also significantly improved after PPN encapsulation. The con-trolled release of PPN for resveratrol lasted for 11 h due to the comprehensive effect of diffusion and skeleton dissolution, con-forming to Ritger-peppas kinetic equation: lnQ = 0.7097 lnt - 1.692 (R2 = 0.9950). Finally, drug-loaded PPN showed remarkable anti-cancer activities to human BGC823 gastric cancer cells (IC50: 64.06 μg/mL) and HepG2 liver cancer cell (IC50: 56.54μg/mL). The results suggest that this resveratrol-loaded PPN may serve as a promising delivery system for long-term anti-cancer.
